Centrifugal Filter
Amicon Ultra-15 Centrifugal Filters
sample volume 15 mL, regenerated cellulose membrane, MWCO 10…
Transfer Membrane
Immobilon-FL PVDF Transfer Membrane
Transfer Membrane from Merck
1 roll, 27 cm x 3.75 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.
Manufactured byMerck
Overview
About Immobilon-FL PVDF Transfer Membrane
The Immobilon-FL transfer membrane is a polyvinylidene fluoride (PVDF) microporous membrane with a nominal pore size of 0.45 μm. It is designed for binding proteins transferred from a variety of gel matrices. The Immobilon-FL transfer membrane is hydrophobic and offers a uniformly controlled pore structure with a high binding capacity for biomolecules. The membrane offers optimal blotting for proteins with molecular weights greater than 20,000. The Immobilon-FL membrane displays very low autofluorescence across a wide range of excitation/emission wavelengths. This property makes it ideal for blotting applications involving fluorescence-based immunodetection. Immobilon-FL membrane can also be used for standard chemiluminescent or chromogenic detection.
Technical Data
Immobilon-FL PVDF Transfer Membrane technical data
- material
- PVDF membrane; plain filter; white filter
- feature
- hydrophobic
- manufacturer/tradename
- Immobilon
- technique(s)
- dot blot: suitable; electrophoresis transfer: suitable; western blot: suitable
- filter L × W
- 27 cm × 3.75 m
- pore size
- 0.45 μm pore size
- capacity
- 155 μg/cm2 adsorption capacity (insulin); 205 μg/cm2 adsorption capacity (BSA); 300 μg/cm2 adsorption capacity (goat IgG)
- compatibility
- for use with All fluorescence dyes; for use with Amido black; for use with CPTS; for use with Coomassie brilliant blue; for use with Ponceau-S red; for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
- detection method
- chemiluminescent; colorimetric; fluorometric
- shipped in
- ambient
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